| 摘要 |
A cloning vector useful in recombining DNA (i.e. recombinant DNA studies) is made by mutagenesis of a satellite bacteriophage P4 wt or P4 vir1 and subsequent separation and purification of the mutant progeny in a cesium chloride equilibrium density gradient. On such a cesium chloride gradient the mutant is found in a range of densities from 1.35 to 1.42 g/ml at 24 DEG C. with peaks at about 1.42, 1.39, and 1.35 g/ml.
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