| 摘要 |
<p>NEW MATERIAL:A compound shown by the formula (R is H or amino-protecting group). EXAMPLE:7-(Nalpha-t-Butyloxycarbonyl-L-glutaminyl-Nepsilon-carbobenzox y-L-lysyl-Nsigma,omega- dicarbobenzoxy-L-arginyl-Nepsilon-carbobenzoxy-L-lysyl)amino-4-methylc oumarin. USE:A substrate for enzyme. Useful for highly sensitive measurement, e.g., titer, purity, etc. of enzyme. PROCESS:Nalpha-And Nepsilon-protecting lysine is condensed through dehydration with 7- amino-4-methylcoumarin, and Nalpha protecting group is removed selectively. The prepared Nepsilon-protecting lysine-4-methylcoumarin derivative is condensed through dehydration with a side chain- and Nalpha-protecting arginine, Nalpha- and Nepsilon-protecting lysine and Nalpha-protecting glutamic acid successively. The side chain-protecting group is removed selectively, to give a compound shown by the formula. The reaction is preferably carried out at from 0 deg.C- room temperature in a solvent, e.g., DMF, etc.</p> |
