Culture Media and Method of Sterilizing Same

摘要

Disclosed are culture media comprising a gelling agent, germicidal bleach containing a halogen, a halogen-inactivating agent to in activate residual halogen and conventional nutrients. Also disclosed is a method of sterilizing a culture medium without autoclaving same by dissolving a gelling agent in buffered water; adding thereto a germicidal bleach containing a halogen; thereby forming a suspension; heating the suspension to boiling; adding to the stirred and heated suspension an excess of halogen-inactivating agent to neutralize residual halogen and then adding nutrients.