| 摘要 |
PURPOSE:To produce human interferon with high unit prolongably, by transforming a lymph corpuscle derived from a human fetus or a human newborn with Epstein- Barr Virus. CONSTITUTION:A lymph corpuscle separated from the spleen of aborted human fetus or the blood of funis of human newborn is floated in a medium for cultivating an animal cell, Epistein-Barr Virus is added to the medium, and cultivation is carried out in the presence of CO2. Cells are collected by centrifugation, these cells are cultivated in a medium for cultivating the animal cell and transformed to give a cell strain producing human interferon prolongably UNCL. The cell strain producing human interferon prolongably UNCL is inoculated into the medium for cultivating the animal cell, the strain is cultivated under flow of air containing CO2, and human interferon is recovered from the culture solution.
|
