| 摘要 |
PURPOSE:Using a synthetic substrate having one or more substrate specificity of the enzyme to be determined as well as one or more photochemically active spectral sensitization pigments in the molecule, the products of the enzymatic reaction are brought into contact with silver halide, exposed to light and developed to determine the activity of the enzyme in high sensitivity. CONSTITUTION:A nonexo-type hydrolytic enzyme to be determined such as protease, ribonuclease, amylase or lipase is mixed with a synthetic substrate that has at least one of structure A specifically catalyzed by the enzyme and at least of structure B of spectral sensitization pigment and the reaction is effected in a buffer solution. The structure A is, e.g. a peptide bond for a hyrolase or an amine or carboxylic group for a transferase. The structure B is, e.g., cyanin or styril pigment. The product containing the structure B is separated from unreacting substrate and either of the product or the unreacting substrate is spotted on a layer containing silver halide. Then, the layer is exposed to the light of the wave length sensitizing the spectral sensitizer and developed, then the developed silver is measured.
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