发明名称 |
Microcarrier cell culture method. |
摘要 |
Anchorage-dependent cells are grown in agitated microcarrier suspension in which the cells and microcarriers are aggregated by periodically providing a temporary residence of said microcarriers and cells outside the main cell culture reactor agitation zone and in a separate compartment wherein they are subjected to a gentle tumbling action within a confined space having substantially the same environmental conditions as in the main cell culture reactor. |
申请公布号 |
EP0046681(A2) |
申请公布日期 |
1982.03.03 |
申请号 |
EP19810303861 |
申请日期 |
1981.08.25 |
申请人 |
MONSANTO COMPANY |
发明人 |
TOLBERT, WILLIAM ROBERT;HITT, MARY MARGARET;FEDER, JOSEPH;KIMES, RICHARD CHARLES |
分类号 |
C07K14/555;C12M3/02;C12N5/02;C12N9/72;C12P1/00;C12P21/00 |
主分类号 |
C07K14/555 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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