Enzymatic conversion of red cells for transfusion.

摘要

Am composition comprising type 0 erythrocyte free of P1 antigenicity; a method of converting erythrocytes of the B antigen type to erythrocytes of the H-antigen type which comprises:
A. equilibrating said erythrocytes to a pH of 5.7-5.8;
B. thereafter contacting the so-equilibrated erythrocytes with an enzyme for a period sufficient to convert the B antigen in said erythrocytes to the H-antigen;
C. thereafter removing said enzyme from said erythrocytes and
D. re-equilibrating said erythrocytes to a pH of 7.2-7.4.
<??>The specification discloses conversion of the B antigens in human and animal blood to the H antigen (0 cells).