| 摘要 |
NEW MATERIAL:A human plasmatic crystalline alpha2-macroglobulin. Molecular weight : 7.6X10<5> (measured by the gel filtration method with Sepharose 6B). Absorbance : E280<1> nm,1cm 8.3(25 deg.C, 0.05M phosphoric acid buffer solution, pH7.0). Isoelectric point: pH5.3. USE:A protease inhibitor, having improved storage stability because of the crystallinity, and capable of inhibiting the abnormal increase in protease activity due to the inflammation or pancreatitis of a living body and preventing the decomposition of peptide hormone or enzyme, etc. by the contaminant protease on purification by the addition thereto. PROCESS:An alpha2-macroglobulin franction of a human plasma is concentrated by the ultrafiltration to give a human plasmatic crystalline alpha2-macroglobulin. The raw material alpha2-macroglobulin is obtained by separating and purifying a human dried plasma with the gel filtration and the colum chromatography. |
