| 摘要 |
NEW MATERIAL:Carcinostatic substance TF-140. The substance has the following characteristics: appearance, light grey to pale brown powder; melting point, no definite melting point, starts to decompose at 210 deg.C, and vigorously decomposes at >=280 deg.C; elemental analysis, C 22.0-28.0%, H 3.0-3.5%, N 5.0-6.5%; solubility, soluble in water, insoluble in methanol, acetone, chloroform, diethyl ether, etc.; color reaction, positive to Molish reaction, phenol-sulfuric acid reaction, etc., negative to ninhydrin reaction. USE:A carcinostatic agent. The substance inhibits the proliferation of Ehrlich ascites carcinoma of mouse, and has immuno-activating activity. PROCESS:TF-140-producing bacteria belonging to Fusobacterium genus, e.g. Fusobacterium nucleatum TF-031 (FERM-P No.5077), is cultured, and the cultured liquid is mixed with a hydrophilic organic solvent to obtain e precipitate. The objective TF-140 is separated from the water-soluble component of the precipitate. |
