| 摘要 |
NEW MATERIAL:Carcinostatic substance TF-132b having the following characteristics: appearance, light grey to pale brown powder; melting pont, no definite melting point, begins to decompose at 110 deg.C, and vigorously decomposes at >=200 deg.C; elemental analysis, C 35.3-39.5%, H 4.5-5.6%, N 2.8-5.4%; solubility, soluble in water, insoluble in methanol, acetone, chloroform, diethyl ether, etc.; color reaction, positive to Molish reaction, phenol-sulfuric acid reaction, etc., negative to ninhydrin reaction. USE:Carcinostatic agent. It can inhibit the proliferation of the cells of Ehrlich ascites carcinoma of mouse, Ehrlich nodular carcinoma, and sarcoma 180 cancer, and has immuno-activating activity. PROCESS:TF-132b-producing bacteria belonging to Fusobacterium genus, e.g. Fusobacterium nucleatum TF-031 (FERM-P No.5077), is cultured, and the cultured liquid is mixed with a hydrophilic organic solvent to obtain the precipitate. The objective TF-132b is separated from the water-soluble component of the precipitate. |
