| 摘要 |
<p>Detection of biological particles of a particular type (I) in a fluid comprises (a) immersing a substrate with a metal surface in a soln. contg. biological (protein) particles (II), other proteins and salts until a monomolecular protein layer has been adsorbed on the surface; (b) washing the substrate with water to remove unbound protein; (c) immersing the substrate in the test fluid so that (I), if present, is bound to (II); (d) binding fluorescent-labelled biological particles (III) to the immobilised (I); (e) washing with water to remove unbound particles; (f) irradiating the substrate with excitation light; and (g) directing the fluorescent light to a detector while directing the reflected excitation light away from the detector. Appts. comprises (a) a highly reflective substrate with a surface for receiving a sample; (b) a light source directing excitation light towards the sample; (c) a detector for measuring the fluorescence from the sample; and (d) means for directing reflected excitation light away from the detector. Method can be used to detect antibodies, hormones, specific- binding proteins, receptors or antigens in biological fluids.</p> |
