| 摘要 |
<p>Hepatitis B surface antigen (HBsAg) is purified by recovering the extracellular fluid from an in vitro culture of cells liberating HBsAg; sepg. from the cells debris and passing onto an immuno-absorbant column on which hepatitis B surface antibodies (HBsAb) are bonded covalently. The HBsAg is eluted, the antigen salts sepd., and the antigen dialysed in a pharmaceutical medium. The cells used for the in vitro culture are pref. Alexander's cells (ATCC CRL 8024). The HBsAb is pref. derived from a non-human source such as guinea pigs, chimpanzees or partic. goats. The column used is a crosslinked dextran, such as agarose; desorption of the HBsAg is by passage of ammonium or sodium thiocyanate. The final dialysis usually takes place against phosphate buffered saline.</p> |
