| 摘要 |
PURPOSE:To prepare the titled substance useful as a raw material of pharmaceuticals, in high yield, in a short time, by culturing a microbial strain obtained by the mutagenic treatment of microorganisms belonging to Arthrobacter genus, in a medium containing a cholic acid salt. CONSTITUTION:Arthrobacter CA-35 strain is subjected to the mutagenic treatment such as treatment with N-methyl-N'-nitro-N-nitrosoguanidine, ultraviolet irradiation, etc. to obtain strains such as Arthrobacter CA-35-A589-29-32 (FERM-P No.5522), Arthrobacter CA-35-A589-47 (FERM-P No.5523), etc. The strain is cultured in a medium containing a carbon source, a nitrogen source, inorganic salts, etc. assimilable with the strain, and containing a cholic acid salt such as cholic acid alkali metal salt as a substrate. The titled substance can be separated from the cultured product by the removal of the cells, addition of hydrochloric acid, addition of ethyl acetate, etc. |
