VERFAHREN ZUR GEWINNUNG EINES NEUEN LEUCINREICHEN 3,1-S-ALPHA2-GLYKOPROTEINS

摘要

New glycoprotein having the following properties: a sedimentation constant in the ultracentrifuge of 3.1 S+/-0.2; molecular weight 49,600+/-4,000; electrophoretic mobility in the range of the alpha 2-globulins of the human serum; isoelectric range pH 3.8 to 4.1; extinction coefficient E 1 cm, 280 nm=1% 7.0+/-0.2; content of about 77+/-2% of alpha -amino acids with a proportion of 17+/-2% of leucine and about 22% of carbohydrates; specific immunological reaction with antibodies directed against the glycoprotein; which can be isolated from human sera and which may be used for the preparation of antisera enabling the detection and determination of the glycoprotein in body fluids.