Serum vitamin B12 assay and kit therefor

摘要

In a vitamin B12 assay, serum, containing vitamin B12 bound to endogenous binders therefor, is released from such endogenous binders by a releasing agent comprised of a water miscible organic liquid, such as, acetone, reducing agent and cyanide ions. Such release can be effected at room temperature, as compared to prior procedures which required heating to elevated temperatures.