The invention relates to improved double-stranded RNAi constructs (sometimes referred to as "solo-rxRNA") and uses thereof. The construct comprises a structure formed in some aspects of the invention by two identical single-stranded polynucleotides, with the structure having two double-stranded stem regions (each having less than 21 base pairs) and a loop or bulge having about 4 to 11 nucleotides on each strand. The construct is resistant to cleavage by Dicer or other Dicer-like RNase III enzymes and is capable of being loaded into a RISC complex to effect RNA interference. In addition, the nucleotides of the present hairpin constructs may be modified to greatly enhance functionality, such as stability and specificity.
申请公布号
WO2010011346(A1)
申请公布日期
2010.01.28
申请号
WO2009US04326
申请日期
2009.07.23
申请人
RXI PHARMACEUTICALS CORPORATION;SAMARSKY, DMITRY;WOOLF, TOD;SALOMON, WILLIAM;KAMENS, JOANNE;KHVOROVA, ANASTASIA;PAVCO, PAMELA, A.
发明人
SAMARSKY, DMITRY;WOOLF, TOD;SALOMON, WILLIAM;KAMENS, JOANNE;KHVOROVA, ANASTASIA;PAVCO, PAMELA, A.