Vitamin D compounds isotopically substituted at carbon 6 and process for their preparation

摘要

A method for isotopically labeling carbon-6 of vitamin D and 5,6-trans-vitamin D compounds which comprises reducing the corresponding 6-oxo-cyclovitamin with a tritio- or deutero-borohydride and subjecting the reduction product to acid-catalyzed solvolysis. The 6-labeled compounds find application in the elucidation of vitamin D metabolism and function and in vitamin metabolite analyses.