<p>A stable and water-insoluble immobilized lactase having a high lactose-hydrolyzing activity is produced with industrial advantages by immobilizing a lactase derived from Aspergillus oryzae on a macroporous phenol-formaldehyde anion-exchange resin. The immobilized lactase can be used in the continuous enzymatic hydrolysis of lactose in milk, whey and whey parmeate.</p>