METHOD OF DETERMINING LIPASE ACTIVITY USING A NOVEL TRIGLYCERIDE REAGENT AND METHOD FOR PREPARING THAT REAGENT

摘要

An improved method of determining lipase activity in a sample by reacting the sample with a triglyceride emulsion and measuring the rate at which the triglyceride is hydrolyzed into fractions by the lipase in which the emulsion is formed by providing, as a reagent, a solid matrix of an excipient uniformly impregnated with the trigylceride, and the solid matrix is dissolved in an aqueous reaction mixture to release the triglyceride. The reagent is prepared by forming a solution of the triglyceride with a solvent, mixing the solution with an excipient and evaporating the solvent to leave a solid matrix of the excipient impregnated with the triglyceride.