| 摘要 |
A method for the determination of one or more components of an immunological conjugate, e.g., antigens, of a fluid sample in a competitive or sandwich technique in which the conjugate is labelled and separated from its reactive mixture by reversible attachment to a solid surface. In a preferred embodiment, the solid surface comprises insolubilized sugar which reversibly bonds to a lectin covalently bonded to one member of the conjugate. After separation of such solid surface from the remainder of the reaction mixture, the insolubilized sugar-lectin bond is broken by contact with a sugar solution which displaces the labelled lectin compound. The immunological components including label and lectin may be preincubated in a homogeneous solution prior to reversible attachment to the sugar solid surface. For a competitive system, a sample containing antigen is incubated with a known quantity of labelled antigen and lectin-bound antibody. In the sandwich technique, the sample antigen is incubated with lectin-bound antibody and further with labelled antibody and this reaction mixture is contacted with insolubilized sugar. Either the competitive or sandwich technique are adaptable to a sequential flowthrough system with sufficient residence time to eliminate the preliminary incubation steps.
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