Process for the preparation of CoA-SPC from Bakers' yeast

摘要

A process for preparing CoA-SPC Bakers' yeast extract which comprises: lysing Bakers' yeast cells; separating the Bakers' yeast cell lysate into solid and supernatant fractions wherein said solid fraction is substantially free of t-factor; treating said solid fraction to solubilize insoluble proteinaceous material other than the insoluble CoA-SPC; separating said solubilized proteinaceous materials from the fraction containing said insoluble CoA-SPC; and contacting said fraction containing said insoluble CoA-SPC with said supernatant fraction containing t-factor to produce soluble CoA-SPC.