HEPATITIS B CORE ANTIGEN

摘要

HBcAg is prepared by isolating Dane particles by isopycnic banding of fluid from HB?Ag positive donors, optionally but preferably pelleting the Dane particles, and then removing the surface antigen by contacting the Dane particles with a nonionic surfactant having from about 15 to about 35 oxyethylene units in the presence of a reducing agent such as mercaptoathanol. The isolated core antigen is used as a diagnostic and immunologic agent.