| 摘要 |
PURPOSE:To obtain an enzyme sensor high in the measuring accuracy with a simple construction, by providing a means in which a potential generated between an enzyme sensitive section and a reference electrode section is received using a load of a low impedance and converted into a current to be detected. CONSTITUTION:Leads 4a and 4b are connected to two iridium oxide layers 2a and 2b formed on the surface of glass 1 by a sputtering method using conductive bonding materials 3a and 3b and a connection part is insulated with an insulating bonding material 5. Then, a liquid of urease dissolved is applied on the iridium oxide layer 2a and after a drying in the air, a glutaric aldehyde solution is dropped into the liquid to form a urease supported enzyme immobilized layer 6 by crosslinking reaction. The other iridium oxide layer 2b is covered with an albumin layer 7 carrying no urease. A resistance 8 of 100OMEGA-10MOMEGA is connected to the leads 4a and 4b thereby enabling production of a sensor quick to respond with a reduced drift. |
