METHOD FOR THE QUANTITATIVE ENZYMATIC DETERMINATION OF ADP AND REAGENT FOR THIS METHOD

摘要

1. Process for the quantitative enzymatic determination of adenosine diphosphate (ADP) in aqueous solution by phosphorylation with a kinase in the presence of a phosphorylated substrate thereof, with the formation of ATP and dephosphorylated substrate, characterized in that the dephosphorylated substrate, possibly with the interpolation of a further adjuvant reaction, is reacted with NAD(P) in the presence of a dehydrogenase and the NAD(P)H forme is measured directly or is reacted with a tetrazolium salt in the presence of an electron carrier, with the formation of a formazane and the latter is measured.