Regenerable insoluble support for protein immobilization

摘要

To permit regeneration of an insoluble support for immobilized proteins, the protein is connected to the support by a spacer arm containing a diazo linkage and when the protein is denatured, the spacer arm is broken by reducing the diazo linkage to remove the denatured protein. New native protein of the same or of a different type is joined to the arm to permit reuse of the support. A preferred support containing a spacer arm is p-(N-acetyl-L-tyrosine azo)benzamidoethyl agarose. A protein is coupled to the carboxyl group of N-acetyl-L-tyrosine and when the protein is spent, the azo bond is reduced to remove the protein and leave p-aminobenzamido agarose which is diazotized and recoupled to N-acetyl-L-tyrosine which is then coupled to fresh protein.