OXIDIZING PHENOLIC COMPOUNDS IN ENZYMATIC IMMUNOASSAY

摘要

An assay for the quantitative determination of entities selected from immunocens and antibodies, which comprises: a. preparing antibodies specific to the entity to be determined, b. preparing an antigen-peroxidase conjugate or an antibody-peroxidase conjugate specific to said entity, c. preparing a system containing a quantity of peroxidasetagged antigen or peroxidase-tagged antibody proportional to the quantity of the entity which is being determined. d. oxidizing a phenolic compound emitting light when subjected to enzymatic oxidation by means of a peroxycompound in the presence of the tagged substance. e. measuring the light emitted and thus establishing the quantity of the said entity, a kit for carrying out such assay and a device for the quantitative determination of immunogens and antibodies by the above assay.