| 摘要 |
The present invention relates to a method for the quantitative determination of enzymes in human and mammal body fluids and tissue extracts, using a substrate which has the structure R1-X-Y-Z-NH-R2 wherein X represents a group having the formula <IMAGE> in which R3 is a straight or branched alkyl radical having 1 to 7 carbon atoms or a cyclohexyl or cyclohexylmethyl radical, Y represents a seryl group or a group having the formula -NH-(CH2)n-CO- in which n is an integer from 1 to 7, Z represents an arginyl or lysyl group, R1 represents hydrogen or an acyl or sulfonyl group and R2 represents an aromatic hydrocarbon radical which optionally may carry substituents. The method includes measuring by photometric, spectrophotometric or fluorescence-photometric methods the quantity of the split product NH2R2 formed by the hydrolytic action of the biologically active factors on the substrate.
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