| 摘要 |
PURPOSE:A methylglyoxal-resistant strain belonging to Escherichia coli and being capable of producing glutathion is cultured in a medium to accumulate glutathion, which is collected in high yield. CONSTITUTION:An above-cited methylglyoxal-resistant strain, e.g., a methylglyoxal-resistant strain (FERM-5279) derived from Escherichia coli B wild strain (ATCC 23226), is cultured in a medium containing 0.2-10% of glucose as a carbon source and 0.5-1% of ammonium sulfate as a nitrogen source. Methylglyoxal is added to the medium by about 0.7-0.4mM and the methylglyoxal resistant strain is inoculated and cultured at 25-35 deg.C and 6-7.5pH under aerobic conditions to accumulate glutathion. After cultivation, the bacterial cells are removed from the culture medium by filtration or the like and the filtrate is subjected to a well-known separation process such as treatment with ionexchange resin to collect glutathion. |
