| 摘要 |
<p>PURPOSE:To prepare the objective compound from alpha, alpha-disubstituted carboxylic acids and optically inactive cephalosporin analogues in the presence of microbials belonging to Pseudomonas genus, etc. and capable of synthesizing cephalosporin analogues. CONSTITUTION:Bacterial strain belonging to xanthomonas genus, Pseudomonas genus, etc. is cultured, and the cultured liquid or the separated bacterial cells are added to a substrate siution containing 0.5-50mg, based on 1ml of the reaction liquid, of an alpha, alpha-disubstituted carboxylic acid of formula I (R1 is six-membered carbon ring residue, etc.,; X is H, etc.) or its reactive derivative, and about 0.1- 50mg, based on 1ml of the reaction liquid, of a cephalosporin analogue of formula II (R2 is H, etc.; Hal is halogen, the configuration of the 6-and 7-H atoms is cis). The amount of the bacterial cells added to the liquid is about 1-50mg (dry basis) 1ml of the reaction liquid. After the reaction, the objective product is separated by conventional process.</p> |
