| 摘要 |
<p>A method for the recovery of creatinine iminohydrolase from an aerobic microorganism is carried out by… (a) forming an aqueous suspension of microbial cells containing creatinine iminohydrolase,… b) disrupting the microbial cells in the suspension to release the creatinine iminohydrolase from the cells, and… (c) before, during, or after step (b) and prior to removal of disrupted microbial cells and other cellular components, introducing a water-miscible organic solvent into the suspension to form a mixture of the organic solvent and the creatinine iminohydrolase-containing suspension. …<??>The creatinine iminohydrolase is retained in the liquid phase of the mixture formed in step (c) while undesired cellular components, such as other microbial cell proteins, are precipitated.</p> |
