摘要 |
Determination of a ligand or receptor is performed in an assay for the ligand or receptor, in which assay a labelled component is necessary, the labelled component being a conjugate between i) a ligand or a receptor and ii) a primary enzyme which is itself capable of producing or removing a modulator for a secondary system, or which is the first enzyme in an enzyme system which can produce or remove a modulator for a secondary system, and that part of the labelled component which is to be determined is determined by allowing the primary enzyme and any other enzymes in the enzyme system to produce or remove the modulator for the secondary system, the secondary system being allowed to function in the presence or absence of the modulator, and a product of the secondary system being determined.
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