摘要 |
PURPOSE:To prepare an acyl-coenzyme A efficiently, by cultivating an acyl-coenzyme A producing bacterium belonging to the genus Macrophomina, Cladosporium, etc. in a medium. CONSTITUTION:An acyl-coenzyme A producing bacterium, belonging to the genus Macrophomina, Cladosporium, Aspergillus, Monascus, Saccharomyces, or Arthrobacter is cultivated in a medium. The prepared culture substance is extracted to give an acyl-coenzyme A.cooxydase, which is converted to a solution containing a crude acyl-coenzyme A.oxidase. The solution is subjected to isolation, e.g., using a wellknown protein, etc., or purification to give a purified acyl-coenzyme A oxidase. In the form of the cultivation, either liquid or solid culture may be good, but preferably the bacterium is inoculated on the liquid medium and subjected to aerated cultivation with stirring from the industrial point of view.
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