Method for the determination of alpha -amylase

摘要

Novel method for the determination of alpha -amylase which method comprises contacting a sample suspected of containing alpha -amylase with a starch derivative carrying a substituent capable of dyestuff-forming coupling; separating from the resulting starch-containing phase the low molecular weight soluble fission products formed by splitting of said starch derivative by alpha -amylase; coupling the said couplable substituent with another dyestuff forming component; and measuring the dyestuff so formed as a measure of the initial alpha -amylase content.