Method of detecting and quantitating haptens and antigens.

摘要

The method comprises contacting a sample containing an unknown quantity of a first immuno-reactive substance selected from haptens, antigens and mixtures thereof, to be detected and quantitated, with (a) a known quantity of a reagent containing a known concentration of an antibody specitic for that first immuno-reactive substance, and (b) a solid phase having a known concentration of a second immuno-reactive substance selected from haptens, antigens and mixtures thereof reactible with the antibody to be passively adsorbed to the solid phase. The contacting is effected for a time sufficient to cause the first and second immuno-reactive substances to combine competitively with the antibody. The solid phase is then isolated, bearing with it the second immuno-reactive substance and the antibody bound thereto. That antibody is then labeled by any suitable means and thereafter the concentration of the labeling bound to the antibody is determined as a measure of the concentration of the antibody bound to the solid phase and as a measure of the first immuno-reactive substance initially present in the sample. The sample and the reagent containing the antibody are in the liquid phase and the sample and reagent may either be mixed together and reacted before contacting the solid phase or, alternately, the sample, reagent and solid phase may contact each other simultaneously for the first time. An enzyme or a radioactive material or the like may be used as the labeling agent. The method has improved sensitivity and adaptability does not require the use of dangerous or expensive reagents.