PROCESS AND COMPOSITION FOR THE QUANTIFICATION OF GLYCEROL AND TRIGLYCERIDES

摘要

A method is described for the assay of glycerol(as either free glycerol or a fatty acid ester of glycerol) in aqueous liquids such as blood serum. The method comprises the steps of I contacting in the presence of an electron acceptor (a) a sample to be assayed and (b) a novel reagent composition comprising 1. optionally, a lipase which hydrolyzed triglycerides to glycerol; 2. glycerol kinase; 3. adenosine triphosphate; 4. .alpha.-glycerophosphate oxidase to produce a detachable change in the presence of triglyceride or a general positive sample; and II detecting the occurrence of said detachable change. The lipase is included when fatty acid esters of glycerol (i.e., triglycerides) are to be detected. Free glycerol from whatever source can be detected with a composition comprising 2-4 above. According to a preferred embodiment, the electron acceptor is oxygen and the reagent composition also includes a hydrogen peroxide indicator composition, i.e., reagents which interact with hydrogen peroxide to produce a detectable product.