WATER-INSOLUBLE ENZYME COMPOSITIONS

摘要

An improved process for preparing a water-in-soluble enzyme composition having a high enzymatic activity and containing the enzyme covalently bonded to an inorganic support material is disclosed, wherein such an inorganic support material being capable of covalently bonding the enzyme, which has a pore-size distribution wherein the most frequent pore-diameter is such that in the resulting enzyme composition for any enzyme concentration contained therein the enzymatic activity per weight unit of the composition is the highest activity per weight unit of the composition which is obtainable at this enzyme concentration, is contacted with an enzyme solution comprising such an amount of enzyme, which is sufficiently low that the enzyme which is bonded in the resulting enzyme composition has a specific activity which substantially is about the same as that of the enzyme in the free state. The support material suitably comprises a SiO2-gel of appropriate pore-size distribution.