Assay method and compounds

摘要

The present invention provides an assay method for opioid activity comprising the steps of: (a) incubating together an opiate receptor material, a radio-iodinated structural analogue of leucine-enkephalin or methionine-enkephalin, and a liquid sample; (b) measuring the percentage inhibition of the binding of the radio-iodinated compound to the opiate receptor material; and (c) determining the opioid activity of the liquid sample using the percentage inhibition measurement. Also provided are a test kit of value in the performance of such assays, and novel compounds of use in such assays together with methods for their preparation.