SETT ATT ODLA OCH/ELLER FESTA CELLER I ETT ODLINGSMEDIUM INNEHALLANDE PARTIKELFORMIGA MIKROBERARE

摘要

The invention relates to a method of cultivating cells in a culture medium, containing particulate microcarriers. The microcarriers used comprise spherical particles of a water-insoluble, but water-swellable cross-linked organic macromolecular substance, said particles exhibiting at their surfaces covalently bound polypeptides capable of binding fibronectin biospecifically. In a water-swollen state the particles have a density within the range of 0.95 - 1.20 g/ml, preferably 1.00 - 1.10 g/ml.The mean size of the particles lies within the range of 100- 500 mu m.