摘要 |
A method of detecting and quantitating the migration of leucocytes in agar gel by (1) fixing the cells; (2) staining them with Ponceau S or a similar dye; (3) destaining; and (4) dissolving the gel by gentle heating in the destaining solution. The stained cells remain adhered to the glass surface of the culture dish, and the cells can be read through a projection microscope or photographed for a permanent record.
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