Hydroxy-succinimide ester compounds

摘要

New hydroxy-succinimide ester compounds of the formula <IMAGE> wherein X is selected from a carboxy-succinimide ester group and radicals of the general formulae: CONHCH2CH(OR' )2 <IMAGE> COOCH2CH2NHCON(R' )2 ; A is -O-CH2-CH2 when n is 2 and X is a succinimide ester group but otherwise is a single bond; n is a whole number of from 2 to 7; m is 1, 2, 3 or 4, R is hydrogen, methyl, or cyanide; and R' is methyl or ethyl. have been found outstandingly useful as bridge-building compounds for linking proteins, especially biologically active proteins, to solid or liquid carrier matrix materials, thereby yielding valuable carrier-bound enzymatically active protein compositions.