| 摘要 |
An electrophoretic technique for assaying the relative distribution of lactate dehydrogenase (LD) isoenzymes characterized in that the electrophoretic and/or substrate buffer employed therein comprises a first and second moiety wherein the first moiety is selected from a group consisting of alkali metal 5,5-diethylbarbiturate, ammonium 5,5-diethylbarbiturate, and mixtures thereof, and wherein the second moiety is selected from a group consisting of bicine (N,N-bis(2-hydroxyethyl)glycine), tricine (N-tris(hydroxymethyl)methylglycine), and glycylglycine. The buffer has a pH of from about 7 to about 9 at 25 DEG C. Preferably, the buffer further comprises a solid organic water soluble acid and either 2-amino-2-methyl-1,3-propanediol (AMPD) or 2-amino-2-hydroxymethyl-1,3-propanediol (Tris) or mixtures thereof. Buffers within the scope of the instant invention, when compared to prior art buffers, increase the relative percentage of LD5 and thereby improve the accuracy and reproducibility of the electrophoretic data.
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