发明名称 |
Method of thioacylation peptide sequencing with alcoholysis of thiazolinones |
摘要 |
A sequencing method which exploits the thioacylation degradation of polypeptides and proteins is disclosed. The process involves reaction of the N-terminal amino acid of a polypeptide with an excess of a thioacylating reagent. After sufficient time to insure quantitative coupling and removal of excess reagent, the N-thioacyl polypeptide is subjected to cleavage by acid which affords a 2-substituted-5(4H)-thiazolinone of the N-terminal amino acid. Subsequent addition of a large excess of an aliphatic primary or secondary alcohol, either directly to the cleavage acid or after its removal, yields the corresponding N-thioacyl amino acid ester, a stable compound suitable for chromatographic separation and subsequent detection by contemporary methods of high pressure liquid chromatography.
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申请公布号 |
US4863870(A) |
申请公布日期 |
1989.09.05 |
申请号 |
US19880166551 |
申请日期 |
1988.03.10 |
申请人 |
BIO-AFFINITY SYSTEMS, INC. |
发明人 |
STOLOWITZ, MARK L.;PAAPE, BETH A.;DEVAUX, ANNE E. |
分类号 |
C07K1/12;G01N33/68 |
主分类号 |
C07K1/12 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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