DETERMINATION OF TRANSCOBALAMINS

摘要

A process and test kit for the quantitative determination of the individual and total unsaturated Vitamin B-12 binding capacities of the three transcobalamins TC-I, TC-II and TC-III in serum. The process comprises incubating a serum sample with a solution of 57Co Vitamin B-12, contacting the resulting mixture with a TC-II adsorbent and with a DEAE-cellulose adsorbent for TC-I and TC-III, measuring the total radioactivity adsorbed to said adsorbents, selectively desorbing TC-III from the DEAEcellulose adsorbent by washing with a monopotassium phosphate solution of about 0.05 M and at a pH of about 4.6, measuring the individual radioactivity adsorbed to each of said adsorbents, and calculating the radioactivity that had been washed from the DEAEcellulose adsorbent by said phosphate solution. The resulting four radioactivity values are functions of the total, TC-II, TC-I, and TC-III unsaturated Vitamin B-12 binding capacities, respectively, of the serum tested. The test kit comprises the essential elements for performing the present process.