| 摘要 |
An improvement in a method for determining a substance present in a biological fluid by oxidizing a compound produced in the course of the determination in the presence of a dehydrogenase enzyme with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide in an amount proportional to the content of the substance in the fluid, which fluid also contains an endogenous material which or a derivative of which produced during the determination likewise is oxidized in the presence of the enzyme with the simultaneous production of reduced beta-nicotinamide adenine dinucleotide, thereby interfering with the determination, which improvement involves first carrying out the oxidation-reduction reaction of the endogenous material or its derivative, then oxidizing the resulting reduced beta-nicotinamide adenine dinucleotide in the presence of lactate dehydrogenase with the simultaneous reduction of pyruvate to lactate, thereafter inhibiting the lactate dehydrogenase enzymatic activity, and thereafter producing a quantity of the aforesaid compound produced in the course of the determination for conducting the oxidation-reduction reaction therewith without interference caused by the endogenous material or the lactate dehydrogenase. Substances which may be determined include alpha-amylase, transaminases, and triglycerides. A two-reagent combination is employed for the determination.
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