| 摘要 |
Methods and materials for quantitative detection of MB and BB isoenzymes of creatine kinase by radioimmunoassay competitive displacement techniques involving antibodies to human BB isoenzyme which are specific for the B monomer, react with BB CK and cross-react with MB CK, but do not cross-react with MM CK. An acylating agent is employed to radioactively label purified CK isoenzymes used as antigens. Incubation is preferably carried out in a Tris buffer having a pH of about 7.4, in the presence of a suitable organic reducing agent such as mercaptoethanol.
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