摘要 |
<p>Tripeptidic derivatives are used for the quantitative determination of proteolytic enzymes, particularly enzymes of the class E.C. 3.4.21. These tripeptidic derivatives are compounds of the formula H-D-X-Y-Z-R, wherein X is one of the cyclohexylglycyl, cyclohexylalanyl, p-hydroxyhexylalanyl, phenylglycyl phenylalanyl, tyrosyl, leucyl, isoleucyl, norleucyl, valyl, norvalyl, (Alpha)-aminobutyryl, alanyl, prolyl or pipecolinoyl groups; Y is one of the cyclohexylglycyl, cyclohexylalanyl, p-hydroxycyclohexylalanyl, phenylglycyl, phenylalanyl or tyrosyl groups and, in as much as X is restricted to the cyclohexylglycyl, cyclohexylalanyl, p-hydroxycyclohexylalanyl, phenylglycyl, phenylalanyl or tyrosyl group s, the symbol Y may represent a leucyl, isoleucyl, norleucyl, valyl, norvalyl, (Alpha)-aminobutyryl, alanyl, prolyl or pipecolinoyl group; Z is an arginyl or lysyl group; and R is a chromogenic group, which may be eliminated by enzymatic hydrolysis and which is susceptible to produce a colour or fluorescent compound. The tripeptidic derivatives mentionned are used preferably in the form of the salts thereof with acids. </p> |