发明名称 |
Competitive enzyme-linked immunoassay |
摘要 |
A competitive enzyme-linked immunoassay for a soluble analyte in which the analyte in the unknown sample and the same analyte attached to a solid phase (immunosorbent) compete for a selected quantity of a soluble first antibody to the substance; a second or bridging antibody is then linked to any first antibody linked to the immunosorbent as a result of the competitive reaction, following which a soluble enzyme/antibody immune complex is linked to bridging antibody on the immunosorbent. The enzymatic activity associated with the thusly-formed enzyme-immunosorbent complex or with the non-immunosorbent fraction of enzyme is measured to determine the presence and/or concentration of analyte in the unknown sample.
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申请公布号 |
US4235960(A) |
申请公布日期 |
1980.11.25 |
申请号 |
US19770820286 |
申请日期 |
1977.07.29 |
申请人 |
MEDICAL COLLEGE OF WISCONSIN THE |
发明人 |
SASSE, EDWARD A;YORDE, DONALD E |
分类号 |
G01N33/543;(IPC1-7):G01N31/14 |
主分类号 |
G01N33/543 |
代理机构 |
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主权项 |
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地址 |
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