FREMGANGSMAATE VED FREMSTILLING AV GAMMA-GLOBULINER

摘要

A gamma globulin-rich fraction from conventional ethanol fractionation of whole placenta is treated (I) WITH 17% ETHANOL AT PH 5.1 (itself a conventional step in the fractionation) (II) THE PRECIPITATE IS DILUTED WITH WATER AND DIALYSED TO REMOVE ALCOHOL, THEN LEDA (2-ethoxy-6, 9-diaminoacridine lactate) is added and the precipitate preferably at pH 7.7 +/- 0.5 discarded (III) AMMONIUM SULPHATE AT APPROXIMATELY SEMI-SATURATION IS ADDED AND THE PRECIPITATE COLLECTED.