发明名称 |
Protease deficient Caulobacter host cells |
摘要 |
Caulobacter optimized for use in expression of heterologous peptides as part of a hybrid Caulobacter S-layer protein are provided as well as methods for producing such Caulobacter. Caulobacter of this invention are deficient in a native Caulobacter protease that cleaves hybrid S-layer proteins. Also provided are Caulobacter libraries and methods for sorting such libraries using cell sorting technology such as FACS.
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申请公布号 |
US7270993(B2) |
申请公布日期 |
2007.09.18 |
申请号 |
US20040478676 |
申请日期 |
2004.05.27 |
申请人 |
THE UNIVERSITY OF BRISTISH COLUMBIA |
发明人 |
SMIT JOHN;NOMELLINI JOHN F.;BINGLE WADE H. |
分类号 |
C12N15/00;C12N15/09;C12N1/21;C12N9/52;C12N15/10;C12N15/57;C12N15/62;C12N15/74;C12P21/02;C12Q1/02;C12Q1/04;C40B40/02 |
主分类号 |
C12N15/00 |
代理机构 |
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代理人 |
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主权项 |
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地址 |
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