PIKE-A ENHANCES INFECTION BY MYXOMA VIRUS

摘要

Cellular Akt activation is increased by transducing an Akt-containing cell with a vector that expresses exogenous PIKE-A (PIl 3 -kinase enhancer activating Akt) in the cell. Expression of PIKE-A increases the susceptibility of cancer cells to myxoma virus infection. Cancer is treated by administering a vector that infects cancer cells and expresses exogenous PIKE-A; and a myxoma virus. Cancer is treated by taking cancer cells from a subject, transducing them ex vivo with a vector that expresses exogenous PIKE-A in the cells, treating the transduced cells with a myxoma virus, and then returning the transduced cells to the subject. Alternatively, myxoma virus can be administered to the patient after the transduced cells have been returned to the patient. The susceptibility of cells to infection with a myxoma virus can be determined by assaying for PIKE-A expression. Cellular expression of PIKE-A in the cell indicates susceptibility to myxoma virus infection.